Historical Introduction Important Notes
1. Scientists and their contribution
2. Staining
Historical Introduction Short Answers
Question 1. Koch’s postulates/Robert Koch?
Answer:
. Koch’s postulates/Robert Koch
- Robert Koch is known as the father of medical microbiology.
- His contributions to microbiology are:
1. Robert Koch Introduced
- Methods of obtaining bacteria in pure cultures using solid media.
- Staining techniques for bacteria
- Methods for isolation of pure strains of bacteria.
2. Robert Koch Discovered
- Anthrax bacillus
- Tubercle bacillus
- Cholera vibrios.
Read And Learn More: Microbiology Question and Answers
3. Robert Koch Proposed Koch’s Postulates
- Koch’s postulates:
- According to Koch’s postulates, a micro-organism can be accepted as the causative agent of an infectious disease only if
- The organism is constantly associated with the lesions of the disease
- If it is possible to isolate the organism in pure cultures from the lesions of the disease.
- Inoculation of such pure culture into suitable laboratory animals will reproduce the lesions of the disease.
- If it is possible to re-isolate the bacterium in pure culture from the lesions produced in the experimental animals.
- According to Koch’s postulates, a micro-organism can be accepted as the causative agent of an infectious disease only if
4. He demonstrated Koch’s phenomenon through his observation of guinea pigs
- It is as follows
- Koch’s phenomenon:
- Robert Koch observed that guinea pigs already infected with tubercle bacillus responded with an exaggerated inflammatory response when injected with the tubercle bacillus (or) its protein.
- This hypersensitivity reaction is known as Koch’s phenomenon.
- Koch’s phenomenon:
Question 2. Louis Pasteur.
Answer:
Louis Pasteur
- He was a trained chemist in France.
- He is known as the father of microbiology.
Important contributions in microbiology:
- He developed methods and techniques of bacteriology.
- Introduced sterilization techniques.
- Developed steam sterilizer, autoclave, and hot air oven
- Developed live attenuated vaccine for prophylactic use
- He studied
- Chicken cholera
- Anthrax
- Hydrophobia
- He also coined the term vaccine.
- He proved that all forms of life even microbes arose only from their like and not de novo.
- He proposed a microbial theory of fermentation to explain the role of microorganisms in disease production
- He controlled the diseases of silkworm